Microsatellite loci were isolated from the aphid parasitoid Lysiphlebus testaceipes (Hymenoptera: Braconidae). Ten loci were obtained from an enriched partial genomic library. Genetic diversity was analysed at seven of these loci and two natural populations, one on oleander and one on citrus. The observed number of alleles ranged from one to 17, and the observed heterozygosity ranged from 0.37 to 0.82. In both populations, no departure from Hardy-Weinberg equilibrium was detected except for one locus. The differentiation between the two populations was characterized by an FST Of 0-09. Lysiphlebus testaceipes (Aphidiidae) Techniques; Genetic techniques; Biochemistry; Genetics; Evolution; Variation; Land zones; Palaearctic region; Eurasia; Europe Lysiphlebus testaceipes; Population genetics techniques; Isolation; characterization & genetic diversity assessment; Microsatellite DNA markers; Nucleic acids; DNA; Molecular genetics; Population genetics; Genetic diversity; Biochemical variation; Microsatellite DNA variation; France; South east none