We describe and illustrate three new bee species of the genus Chilicola Spinola that occur at high altitudes in the Eastern Andes of Colombia: C. (Anoediscelis) paramoides Gonzalez sp. nov., C. (Hylaeosoma) bochica Gonzalez sp. nov., and C. (Oroediscelis) deborahae Gonzalez sp. nov. We also provide biological notes and discuss the phylogenetic relationships of C. deborahae; nests of this species were found inside dry flower sterns of Espeletia argentea Humb. & Bonpl. (Asteraceae) and a palynological analysis showed that 80 % of the pollen grains found in brood cells belonged to Arcytophyllum aff. nitidum and A. muticum (Rubiaceae). The ichneumonid wasp Grotea sp. is also recorded parasitizing brood cells of C. deborahoe. BR Systematics. Nomenclature. New taxa. Parasites diseases and disorders. Parasites. Insect parasites. Hosts. Insect hosts. Land zones. Neotropical region. South America. Chilicola (Anoediscelis) paramoides Gonzalez 2009 (Colletidae): Sp nov, Colombia, P. 146; Chilicola (Hylaeosoma) bochica Gonzalez 2009 (Colletidae): Sp nov, Colombia, P. 148; Chilicola (Oroediscelis) deborahae Gonzalez 2009 (Colletidae): Sp nov, Colombia, P. 150, Host; Grotea (Ichneumonidae): Parasite. Chilicola (Anoediscelis) paramoides, Chilicola (Hylaeosoma) bochica: Colombia, New species; Chilicola (Oroediscelis) deborahae: Hymenopteran parasites, Grotea, New record, Colombia, New species; Grotea: Hymenopteran hosts, Chilicola (Oroediscelis) deborahae, Recorded from new host species, Colombia. ST Animalia, Arthropoda, Insecta, Hymenoptera, Apocrita, Aculeata, Apoidea, Colletidae; Animalia, Arthropoda, Insecta, Hymenoptera, Apocrita, Terebrantia, Ichneumonoidea, Ichneumonidae. Arthropods; Hymenopterans; Insects; Invertebrates. none