This work reports the occurrence of parasitoid attacks on pupae of flies in urban and woody areas of Itumbiara, Goias, Brazil. From March 2001 through June 2002, 909 parasitoids were collected from 3,887 pupae of flies in the urban area and 684 were collected from 830 pupae of flies in the woody area in five different substratums: humans feces, fruit, chicken, fish and bovine kidney. The pupae were obtained by the flotation method. The prevalences of parasitoidism in the urban and woody areas were 23.6% e 82.4%, respectively. Parasites diseases and disorders; Parasites; Insect parasites; Hosts; Insect hosts; Habitat; Terrestrial habitat; Man made habitat; Land zones; Neotropical region; South America Muscoidea (Cyclorrhapha); Hymenopteran parasites; Forest and woodland; Urban habitat; Brazil; Goias; Itumbiara; Hymenopteran parasite records & prevalence in woodland & urban habitats Braconidae; Figitidae (Cynipidae ); Chalcididae; Encyrtidae; Pteromalidae; Dipteran hosts; Muscoidea; Records & prevalence in woodland & urban habitats; Forest and woodland; Urban habitat; Brazil; Goias; Itumbiara; Records & prevalence in dipteran hosts in woodland & urban habitats none2004