A study was conducted to assess toxicity of Spinosad 45 SC (Tracer) and other commercial insecticides on natural enemies associated with insect pests of pigeonpea at Pantnagar during kharif season of 2003 at Crop Research Centre of G. B. Pant University of Agriculture & Technology, Pantnagar. Among the natural enemies, spider population appeared to be significant during 50 per cent flowering and podding stage of crop growth period. The insecticides did not affect the natural population of spiders during the crop growth. The natural enemies of pigeonpea pests observed included Mantis religiosa (Linnaeus), Crocothemis servilia Drury, Chrysoperla carnea (Stephens), Eocanthecona furcellata (Wolff.), Apanteles sp., Xanthopimpla sp., Ropalidia ferruginea (Fabricius), Odynerus ovalis Saussure, Clubiona sp. and Araneus sp. The spiders and braconid parasitoids were more prevalent than the other natural enemies. none no species