noneEnter Lance flies Dasiops spp., are responsible for the flower bud drop and fruit damage in sweet passion fruit (Passiflora ligularis Juss.) crop plantations, and cause economic losses to the growers. This study aimed to clarify what species of lance flies are attacking the sweet passion fruit flower buds inthe Southern part of Huila province (Colombia), to evaluate and characterize its damage, to identify its natural enemies present at the crop and finally to evaluate the insecticidal effect of nine aqueous botanical extracts over the survival of Dasiops inedulis adults. In Pitalito, Palestina, Isnos and Gigante (Huila), thirty granadilla farms were sampled and the species D. inedulis was found attacking flower buds and D. yepezi was found attacking fruits. The percentage of bud infestation levels of D. inedulis ranged between 3.87 [plus or minus] 2.78 at Isnos and 24.60 [plus or minus] 5.36 at Pitalito. The most common attacked flower buds size were 2-4 cm long. The sex ratio was 1:1. The natural enemies obtained from the rearing were the parasitoids Aspilota sp., Pentrapia sp., Basalys sp., Pachycrepoideus vindemmiae, and a specie from Eucoilinae subfamily. The best botanical extracts under greenhouse conditions were Hura crepitans (Euphorbiaceae) at 5% (72.5% mortality) and Ricinus communis at 25% (52.5% mortality). In field conditions, the R. communis (Euphorbiaceae) extract at 25% caused a significant higher mortality (40% mortality) compared to the control. Under both, field and greenhouse conditions, the commercial control was always more effective than the botanical extracts tested. BR Parasites diseases and disorders. Parasites. Insect parasites. Hosts. Insect hosts. Habitat. Man made habitat. Land zones. Neotropical region. South America. Dasiops inedulis (Lonchaeidae): Host; Dasiops yepezi (Lonchaeidae): Host; Pachycrepoideus vindemmiae (Pteromalidae): Parasite; Eucoilinae (Cynipidae): Parasite; Aspilota (Braconidae): Parasite; Basalys (Diapriidae): Parasite; Pentapria (Diapriidae): Parasite. Aspilota, Basalys, Eucoilinae, Pachycrepoideus vindemmiae, Pentapria: Dipteran hosts, Dasiops inedulis, Record, Dasiops yepezi, Cultivated land habitat, Passion fruit plantation habitat, Colombia, Huila, south; Dasiops inedulis, Dasiops yepezi: Hymenopteran parasites, Aspilota, Record, Basalys, Pachycrepoideus vindemmiae, Pentapria, Eucoilinae, Cultivated land habitat, Passion fruit plantation habitat, Colombia, Huila, south. ST Animalia, Arthropoda, Insecta, Diptera, Brachycera, Cyclorrhapha, Lonchaeidae; Animalia, Arthropoda, Insecta, Hymenoptera, Apocrita, Terebrantia, Chalcidoidea, Pteromalidae; Animalia, Arthropoda, Insecta, Hymenoptera, Apocrita, Terebrantia, Cynipoidea, Cynipidae; Animalia, Arthropoda, Insecta, Hymenoptera, Apocrita, Terebrantia, Ichneumonoidea, Braconidae; Animalia, Arthropoda, Insecta, Hymenoptera, Apocrita, Terebrantia, Proctotrupoidea, Diapriidae. Arthropods; Hymenopterans; Insects; Invertebrates; True Flies.