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  • In vitro culture of the ectoparasitoid Bracon greeni Ashmead
  • 作者: Xie, Z.N.; Li, L. and Xie, Y.Q
  • literature id: 49053
  • catalog nub: TPL_XIEnnn1989IVCOT43304370
  • 文献库: Taxapad收录文献
  • type: article
  • publication name: Acta Entomologica Sinica
  • publish date: 1989-01-01
  • pages: 433-437
  • volume: 32
  • issue: 4
  • 创建时间: 2021-03-02 15:00:33
  • create by: zxmlmq (admin)
  • comment:

    none The larval ectoparasitoid Bracon greeni Ashmead is an effective biological agent for controlling the major insect pest Eublemma amabilis Moore of the lac insect Laccifer lacca Kerr. It has been reared in vitro from egg to adult on artificial diets composed of pupal haemolymph of Antheraea pernyi Guerin-Meneville, chicken egg yolk and cow milk suspension (1g milk powder in 10 ml water), added with gentamycin sulfate (400-600 i.u. per ml of diet). The diets were encapsulated inside parafilm stretch wrappers to form the "artificial hosts". The survival rate of larvae was 85-91%, pupation rate 70-71%, and adult emergence rate 67-69%. the egg stage lasted 20-22 hours; duration of larval development was 3-4 days and the prepupa and pupa developed for 7-8 days. The total preimaginal development required 11-13 days at 26+-2 degree C. The body lengths of the reared adults measured 3.2-4.5 mm in female and 2.5-4.0 mm in male. The sex ratio was about 1:3 ( female : male ). It was found that the ectoparasitoids reared in vitro developed with similar rate as those on the larvae of its natural host, and they had bigger body size but inferior female ratio. The mated females produced viable offsprings of both sexes on the greater wax moth larvae as well as on the artificial hosts. The present Work indicates that B. greeni can be mass produced in vitro after some improvements in the rearing technique.

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